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KMID : 1134820190480121366
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 12 p.1366 ~ p.1372
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Biotin Analysis in Selected Agricultural Foods Consumed in Korea
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Kwon Ji-Hyun
Lee Sang-Hoon
Choi Young-Min
Kim Young-Hwa
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Abstract
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Biotin is a water-soluble B vitamin involved in the production of energy and functions as a coenzyme in bicarbonate-dependent carboxylation reactions. In this study, contents of biotin in various foodstuffs consumed in Korea were quantified by immunoaffinity column/high-performance liquid chromatography (HPLC). The linearity of biotin standard was over R2＝0.9999 in the range of 0.015∼0.960 μg/mL. Limits of detection and quantification of biotin were 0.005 and 0.013 ng/μL, respectively. The method showed 100.49% recovery from the certified reference material, SRM (standard reference material) 1849a (infant/adult nutrition formula). In this study, biotin contents of nuts, fruits, vegetables, egg, and edible insects were analyzed. The highest content of biotin in nuts was observed in seasoned peanuts (50.657±0.797 μg/100 g), whereas the contents of biotin in fruits and vegetables were relatively lower, such as 2.672±0.126 μg/100 g in Gaeryangmeoru and 6.891±0.240 μg/100 g in blanched cauliflower. The highest contents of biotin in eggs and insects were 88.842±0.404 μg/100 g in duck egg (yolk, raw) and 121.440±13.326 μg/100 g in silkworm (larva, dried), respectively. These results show that immunoaffinity column/HPLC assay has acceptable sensitivity, accuracy, and precision for biotin analysis. The biotin values presented in this study can be used for a Korean standard food composition table.
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KEYWORD
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biotin, HPLC, immunoaffinity column, Korean standard food composition table
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